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Efficient deletion of Yap and Taz in CNC-derived cells. Hippo signaling activity, indicated by the level of phosphorylated Yap (pYAP) in control embryos (A,B) and in Wnt1Cre; Yapf/f; Tazf/f embryos (C,D). pYAP levels were reduced in CNC-derived cells (white arrows) but not in non-CNC derived cells (red arrows). Hippo-active cells (green) were stained with pYAP; nuclei (blue) were stained with DAPI.
