Fig. 2.

Vascular defects, hemorrhage and neural tube defects following Yap and Taz inactivation using the Wnt1^Cre2SOR Cre driver. Both Wnt1^Cre2SOR; Yap; Taz dCKO (A-D) and Wnt1^Cre2SOR; Yap^f/f; Taz^f/+ (M-P) embryos show lethality at E10.5 with severe vascular defects, hemorrhage and defects in neural tube closure, whereas control embryos (I-L) and Wnt1^Cre2SOR; Yap^f/+; Taz^f/f embryos (E-H) show no obvious defects. (Ma-Pa) Zoom images of branchial arch vessel defects, hemorrhage and neural tube defects of Wnt1^Cre2SOR; Yap^f/f; Taz^f/+ embryos. Three-dimensional imaging using optical projection tomography microscopy indicates that the neural tube of control embryos closed normally (Q), whereas Wnt1^Cre2SOR; Yap; Taz dCKO embryos have a defect in neural tube closure (R).