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. 2016 Feb 1;143(3):530–539. doi: 10.1242/dev.128116

Fig. 6.

Fig. 6.

piRNAs are age modulated. (A) Select transposons are upregulated in aged ovaries. qRT-PCR analysis was used to determine the expression status of TEs in ovaries at 30 days versus 3 days. Mean±s.d., n=3 independent experiments; *P<0.05, **P<0.01, Student's t-test. (B) The length of piRNAs is age modulated. RNA from ovaries of control flies at 3 (newly eclosed adult), 15 (young), 30 (mid-age) and 45 (old) days of age were used to generate age-modulated piRNA profiles. Box plots show that with age piRNAs become progressively shortened from the 3′ end. Fifteen versus 3 days, P=0.001371; 30 versus 3 days, P<2.2×10−16; 45 versus 3 days, P<2.2×10−16; Wilcoxon signed-rank test. (C) The abundance of piRNAs in ovaries becomes slightly decreased with age. Box plots for normalized read counts are shown as log10 value. Fifteen versus 3 days, P=3.182×10−1; 30 versus 3 days, P<2.2×10−16; 45 versus 3 days, P<2.2×10−16; Wilcoxon signed-rank test. (A-C) Flies were of genotype 5905.