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. 2016 Feb 1;143(3):473–482. doi: 10.1242/dev.133843

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Increased expression of CD44 from the first to the second trimester and the spatiotemporal pattern of CD44 protein expression. CD44 gene (A; *P<0.05, n=3) and protein (B-G) expression analyses reveal the distinct temporal and spatial patterns of CD44⁺ cells (red) during human semilunar valvulogenesis. DAPI is in white. The green lines highlight the semilunar cushions. OFT, outflow tract; f, fibrosa; v, ventricular side; CC, cardiac cushions; an, leaflet annulus; asterisks, erythrocytes. Scale bars: 200 µm in B-E; 400 µm in F,G.