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. 2016 Feb 15;143(4):715–727. doi: 10.1242/dev.128090

Fig. 6.

Fig. 6.

C-cadherin localization on lamellipodia depends on RLC. Actin is red and C-cadherin or caC-cadherin is green. (A,B) Cadherin forms puncta (arrow) on lamellipodia (A) that resolve into long plaques (arrow), linking actin cables from neighboring cells (B, asterisk). (C) By contrast, lamellipodia on RLC morphant cells maintain a cloud of diffuse C-cadherin signal (arrow) that does not resolve into puncta. (D) Time-lapse microscopy of C-cadherin dynamics in adjacent control cells labeled singly for actin (left) or C-cadherin (right) display adhesions (arrows) that when broken, snap backwards rapidly (asterisk). (E) RLC MO-treated cells (co-injected with a blue dextran) do not make adhesions with neighboring control cells. (F) pnRLC-expressing morphant cells display little protrusive activity or adhesion remodeling (arrow). (G-I) Cells expressing caC-cadherin exhibit longer linear adhesion plaques than C-cadherin-expressing cells (arrows in G,H), which are associated with thicker actin cables (arrow in G′, quantified in I; n=20 widths per condition). Merged images are in G″,H″. Scale bar in A is 20 μm for all images. Error bars represent s.e.m. ***P<0.001.