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. 2015 Jun 24;5(1):e1062967. doi: 10.1080/2162402X.2015.1062967

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Figure 2. — Soluble form of CD137 predominates over membrane-attached forms. (A) Diagram representing the cDNA with the exons of mouse CD137 and RT-PCR products amplified with the indicated primer pairs. (B) Quantitative RT-PCR analyses using primers that amplify either transmembrane (primer pair 3–4) or total CD137 cDNA (primer pair 1–2) in corresponding cell lines. (C) RT-PCR products of total CD137 cDNA (primer pair 5–6) amplification showing that the soluble CD137 (sCD137) predominates over the transmembrane CD137 (tmCD137) form. (D) Sandwich ELISA assessment of the concentration of sCD137 in the tissue culture supernatants of the CT26 cell line. TM: transmembrane domain; MM: molecular weight marker; N: normoxia; H: hypoxia.