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Soluble CD137 is produced by in vivo grafted tumors. (A) CT26 tumors (10×10 mm in diameters) excised from CD137−/− mice were analyzed by quantitative RT-PCR for mCD137 encoding the transmembrane and the total CD137 isoforms. (B) The concentration of soluble CD137 (sCD137) assessed by ELISA in the sera of Rag−/− mice grafted with 5×105 CT26 cells for 21 days.
