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. 2015 Jul 28;5(1):e1061175. doi: 10.1080/2162402X.2015.1061175

Figure 5.

Figure 5.

IL-17A suppresses proliferation and IFNγ production of CD8+ T cells in vitro. (A) Representative flow cytometry histograms of CD8+ T cells from naive C57BL/6 mice cultured with or without CD11b+Ly6G+ cells isolated from the bone marrow of naive C57BL/6 mice. Proliferation, CD44 expression, IFNγ production of CD8+ T cells was measured by flow cytometry after anti-CD3/CD28 stimulation. (B) Representative flow cytometry histograms of CD8+ T cells from naive C57BL/6 mice cultured with or without CD11b+Ly6G+ cells, isolated from the bone marrow of naive C57BL/6 mice, in the presence or absence of IL-17A, and assayed by flow cytometry as in A. (C) Representative flow cytometry histograms of IFNγ production from CD8+ T cells cultured in the presence or absence of CD11b+Ly6G+ cells isolated from the spleens of LLC tumor-bearing C57BL/6 mice, with or without IL-17A. (D) Quantification of IFNγ production of CD8+ T cells cultured in the presence or absence of IL-17A. Values are mean ± SEM (n = 3).