Figure 7.

The mTOR pathway is involved in neutrophil hypersegmentation. (A) Inhibitory effect of rapamycin on captopril-induced hypersegmentation. Neutrophils were exposed to 500 μM captopril in the presence of PD90859 (10 μM), SB203580 (10 μM), wortmannin (1 μM), and rapamcyin (1 μM). n = 4 for each group; ***p < 0.001 compared to vehicle-treated cells; ###p < 0.001 compared to captopril-treated cells. Results are shown as means ± SEMs. (B) Western blot analysis of phosphorylation of 4E-BP1 and S6K in captopril-treated neutrophils. Neutrophils were treated with 500 μM captopril for 4 h in the presence or absence of 1 μg/mL rapamycin.