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. 2016 Feb 15;129(4):854–867. doi: 10.1242/jcs.183152

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — FAZ-like structures in the L. mexicana flagellar pocket neck. (A) Overview of the key non-membrane structures in the L. mexicana flagellar pocket neck. Dashed lines indicate regions of particular interest, illustrated in B–D. Generated using tomogram PL1. (B–D) Transverse views (looking proximal to distal) of the structure of the flagellar pocket neck at three key points along its length, and 10-nm virtual transverse sections (generated from tomogram volumes), showing the electron density corresponding to the structures segmented. (B) The exit of the flagellum from the flagellar pocket. Tomogram PT2. (C) The central portion of the flagellar pocket neck. Tomogram PT1b. (D) The flagellar pocket collar. Tomogram PT1a. Dashed lines indicate the location of longitudinal sections of particular interest, with example images shown in E to H, as indicated. (E-H) 10-nm virtual longitudinal sections (generated from tomogram volumes), illustrating electron densities corresponding to key structures in the flagellar pocket neck region. E and H are tangential to the flagellar membrane, F and G are perpendicular. Oriented with distal upwards. (E) The double line (arrows) of the flagellar pocket collar. Tomogram PL2. (F) The primary flagellum attachment area. Digitally straightened from tomogram PL3. (G) Example of additional flagellum attachment areas (Att. area) at the flagellar pocket neck lip. Tomogram PL1. (H) The electron-dense FAZ filament and neighbouring electron densities, next to the MtQ. Digitally straightened from Tomogram PL2.