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. 2016 Jan 26;4(2):e12681. doi: 10.14814/phy2.12681

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Characterization of cultured fibroblasts. (A) Fibroblasts growing out of explants from SR and AF samples after 1 week of culture. Scale bar: 100 μm. (B) Staining for the expression of vimentin, human fibroblast surface protein (hFSP), alpha‐smooth muscle actin (α SMA) and CD31 in AF fibroblasts before replating. Nuclei are stained in blue with DAPI. Cells expressing α SMA‐positive myofilaments are easily distinguished from negative cells (arrows). Scale bar: 50 μm. (C) Cell count after 3 weeks of culture. The number of cells was normalized to the number of tissue pieces. *P < 0.05, unpaired Student's t‐test. (D) Staining for Vimentin and hFSP in AF fibroblasts after replating. Nuclei are stained in blue with DAPI. Scale bar: 50 μm.