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. 2016 Jan 26;4(2):e12663. doi: 10.14814/phy2.12663

Figure 5.

Figure 5

Effect of Bmal1‐specific siRNA treatment on Per2‐dLuc oscillations in UESCs. (A) Representative records of bioluminescence showing the effect of Bmal1 interference on the rhythmic expression of Per2‐dLuc oscillations in UESCs. UESCs were treated with Bmal1‐specific siRNA (Bmal1‐siRNA) or nonsilencing RNA (CONT) according to the indicated protocols. The cells were then synchronized with DXM for bioluminescence determination (time: 0 h). Amplitude of Per2‐dLuc oscillation in UESCs was estimated with or without Bmal1 siRNA treatment. (B) According to the first Per2‐dLuc phase (solid line) in panel A, total RNA samples were collected from control and siRNA‐treated cells at the indicated times after synchronization. RT‐qPCR analyses of transcript levels were performed using their specific primers. The transcript levels were calculated and normalized to each value given by the control sample at 30 h. The Cosinor analysis method was used to determine the rhythmic expression of examined genes. Statistical rhythmicity (dotted curves, P < 0.05) and peak time with 95% confidential intervals (line length) are shown in each panel.