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. 2016 Jan 26;4(2):e12663. doi: 10.14814/phy2.12663

Figure 7.

Figure 7

Effect of the REV‐ERB α antagonist SR8278 treatment on Per2‐dLuc oscillations and clock genes expression in UESCs. (A) Representative records of bioluminescence showing the effect of SR8278 on the rhythmic expression of Per2‐dLuc oscillations in UESCs. Cultured UESCs were washed twice with serum‐free medium and subjected to Per2‐dLuc bioluminescence determination with 10 μmol/L SR8278 or 0.1% DMSO (vehicle control) (time: 0 h). Amplitude of Per2‐dLuc oscillation was estimated in the cells with or without SR8278 treatment. (B) According to the first Per2‐dLuc phase, total RNA samples were collected from control and SR8278‐treated cells at the indicated times after synchronization. RT‐qPCR analyses of transcript levels were performed using their specific primers. The transcript levels were calculated and normalized to each value given by the control sample at 26 h. The Cosinor analysis method was used to determine the rhythmic expression of examined genes. Statistical rhythmicity (dotted curves, P < 0.05) and peak time with 95% confidential intervals (line length) are shown in each panel.