Figure 8.
Expression profiles of core Gdf genes in UESCs treated with the REV‐ERB α antagonist SR8278. (A) According to the first Per2‐dLuc phase shown in Figure 7A, total RNA samples were collected from control and SR8278‐treated cells at the indicated times after synchronization. RT‐qPCR analyses of transcript levels were performed using their specific primers. Effect of SR8278 treatment on the expression of Gdf10 and Gdf15 was also performed in the presence or absence of P4. The transcript levels were calculated and normalized to each value given by the control sample at 26 h.