Figure 4.

Treatment with blocking antibodies does not reduce the total number of damaged fibers, but reduces the percentage of damaged fibers that are invaded by inflammatory cells, 2 days after lengthening contractions. Total damaged fibers expressed as a percentage of the total number of fibers in a muscle section (A). Representative partial section of muscle from uninjured mouse (B), uninjected mouse (C), and mouse injected with P/E‐selectin blocking antibodies (D). Damaged fibers expressed as a percentage of the total number of damaged fibers in a muscle section (E). Damaged fibers fell into one of three categories. Category 1 consisted of round and often swollen fibers stained dark with eosin Y. Category 2 consisted of fibers with variable or pale staining with eosin Y. Category 3 consisted of fibers similar to those in category 2, but with several nuclei within the fiber, assumed to be inflammatory cells. *Significantly different from category 1 within experimental group. #Significantly different from category 2 within experimental group. ψSignificantly different from control within category. Significance was determined by a two‐way analysis of variance (P < 0.05) (E). Magnified views of fibers from C and D (F–I). Examples of category 1 (F), category 2 (G, H) and category 3 (I) fibers. Scale bars = 200 μm.