. 2015 Dec 3;3(12):e12638. doi: 10.14814/phy2.12638

Table 2.

Effects of infusion of Gcg‐derived peptide hormones on the first and second phases of insulin secretion from isolated perfused rat pancreas preparations

Peptide infusion	First phase	Second phase
Peptide infusion	First phase	Time	Treatment	Time:Treatment
GLP‐1 7‐36, 30 pmol⁻¹ min⁻¹	0.015	0.0005	NS	NS
rGRPP, 30 pmol⁻¹ min⁻¹	0.009	<0.0001	NS	0.0039
rGRPP, 3 pmol⁻¹ min⁻¹	0.011	<0.0001	NS	0.0063
rGRPP‐LP, 30 pmol⁻¹ min⁻¹	0.019	<0.0001	0.047	0.0006
rGRPP‐LP, 3 pmol⁻¹ min⁻¹	0.044	<0.0001	NS	0.018

Calculated P‐values derived from Welch's t‐tests for first‐phase insulin secretion data, and linear mixed‐effects models (LMEM) of the form (Insulin Secretion ~ [Time + Treatment + Time:Treatment]) fitted by restricted maximum likelihood to data describing the second phase of insulin secretion following infusion of peptide solutions. Time values used for these calculations were at t = 22 min for the first phase and from t = 26 min until the end of the infusion at t = 40 for the second phase. NS, not significant (i.e., P > 0.05) comparing insulin secretion in control pancreases with that in pancreases perfused with hGLP‐1 7–36 (30 pmol min⁻¹), rGRPP (3 or 30 pmol min⁻¹), or rGRPP‐LP (3 or 30 pmol min⁻¹).