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. 2015 Dec 23;3(12):e12656. doi: 10.14814/phy2.12656

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Measurement of integrated electromyographic (iEMG) from gastrocnemius. EMG was measured while the mouse moved freely in its cage. (A) Example of an original EMG trace over a 2‐sec recording. (B) Rectified EMG trace. (C) Calculated iEMG. The rate at which iEMG increased over time (diEMG/dt) varied constantly. The iEMG trace was then divided in segments as shown with the vertical dashed lines. For each segment, we calculated the slope (i.e., diEMG/dt) by linear regression analysis and its duration. Inset above A: example of an EMG trace after the mouse had been anesthetized; a similar trace was obtained if the leg was moved. Horizontal bar: 200 msec; vertical bar: 1 μV.