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Quantitative real‐time PCR analysis of Npy (A), Agrp (B), Pomc (C), and Cart (D) in the hypothalamus after intracerebroventricular injection of ghrelin. CT, control; LP, low‐protein diet. The bar represents the mean ± SEM expressed as relative units of mRNA standardized against Actb (n = 4). **P < 0.01; ***P < 0.001.
