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. 2015 Dec 10;3(12):e12629. doi: 10.14814/phy2.12629

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Western blotting analysis of phosphorylation of AMPK in the hypothalamus after intracerebroventricular injection of ghrelin.(A) A single band in a size of 68 kD for total and phosphorylated AMPK was detected. (B) The relative abundance of total AMPK. (C) The relative abundance of phosphorylated AMPK. (D) The ratio of abundance of phosphorylated to total AMPK. CT, control; LP, low‐protein diet. The bar represents the mean ± SEM expressed as relative abundance of protein standardized against Actb (n = 4) or the ratio of abundance of phosphorylated to total AMPK. *P < 0.05; **P < 0.01.