Fig. 9.

ROS mediates the augmented Ca²⁺ current in IH-treated CaV3.2-HEK cells. A: MDA levels were determined as an index of ROS generation. Average data (means ± SE) of MDA in CaV3.2-HEK cells treated with normoxia or IH₆₀ or IH₆₀ in the presence of 50 μM MnTMPyP (n = 3 independent experiments each). B: representative Ca²⁺ currents from CaV3.2-HEK cells treated with normoxia or IH₆₀, with and without 50 μM MnTMPyP. The recording protocols were same as in Fig. 8. C: average (means ± SE) normalized peak current density obtained with a step to −30 mV for normoxia (n = 9 cells)-, IH₆₀ (n = 7 cells)-, and IH₆₀ + 50 μM MnTMPyP (n = 9 cells)-treated CaV3.2-HEK cells. **P < 0.01; n.s., not significant (P > 0.05).