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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Clin Chem. 2015 Nov 19;62(1):252–261. doi: 10.1373/clinchem.2015.245860

Fig. 3. Comparison of SRM with ALK FISH, IHC, and DNA sequencing in selective cases.

Fig. 3

FISH and paired IHC for patient DH5 to represent FISH+/IHC+ cases; DNA sequence within the MS targeted peptide from DH1 to represent wild-type sequence. (A), ALK FISH testing shows deletion of the 5′ (green) signal with retained 3′ (orange) signal, consistent with rearrangement. Arrows indicate the rearranged red signal. (B), The FISH+, SRM−, and IHC− case (DH9) showed a nonsense point mutation that resulted in a stop codon (p.Q1429X); therefore, nonfunctional fusion protein would be produced.