Table 1.
Characteristics of 26 microsatellite primers developed for Cypripedium japonicum.a
| Locus | Primer sequences (5′–3′) | Repeat motif | Fluorescent labelb | Allele size range (bp) | GenBank accession no. |
| Cypj025 | F: TTCGAGATGCTTCCGACCC | (TTG)9 | VIC | 236–239 | LC73788 |
| R: TTGGCCGAGTTCGTTCGAG | |||||
| Cypj047 | F: TGTCAGTGTCGCTGCCTTC | (GCG)10 | FAM | 191–199 | LC73789 |
| R: AGTTCACGACCCGATTGTC | |||||
| Cypj060 | F: TCACTGAGAGGTGTGATTCC | (AC)12 | FAM | 160 | LC73790 |
| R: CATTGCATGCTTGTGTTGT | |||||
| Cypj061 | F: TTTTGGATCAAATCATCACCT | (AC)10 | FAM | 154–156 | LC73791 |
| R: CTTCTTTAGAGGAAGATCCAAGA | |||||
| Cypj062 | F: TGAGGCTACCAGTTAATGTCTG | (AG)12 | FAM | 131–135 | LC73792 |
| R: ATCTTCCTCTCCACCAATCA | |||||
| Cypj065 | F: ACAAGAACCTGCCAGAAAAC | (AG)10 | FAM | 122–124 | LC73793 |
| R: GACAAGATTTTCAATTCATCACTC | |||||
| Cypj069 | F: GCATCATTCAAGGTGTCAAA | (GA)10 | VIC | 104 | LC73794 |
| R: CTTCCTCCCTCTCTCTTTCC | |||||
| Cypj082 | F: ATTCATAAAACCAGGGCTGA | (GA)11 | FAM | 158–162 | LC73795 |
| R: TCAAAGGATGGTGGAGAAGT | |||||
| Cypj091 | F: TCGATGACATTGATATGGAAG | (GA)23 | FAM | 125–131 | LC73796 |
| R: AGGGATGATCTTTTCCTTCA | |||||
| Cypj094 | F: CCTCAATAGGGACACACACA | (AG)11 | VIC | 128–157 | LC73797 |
| R: AGTTCAATGGAACCCTCAAA | |||||
| Cypj100 | F: GGTGAATTATATGATGGAAGCA | (AC)11 | VIC | 173–177 | LC73798 |
| R: TTGCTGTTATTACTCCCACCT | |||||
| Cypj114 | F: TTAAGGGACTTTCTCTGATTCAAC | (CT)9 | FAM | 240 | LC73799 |
| R: CCAATCACTTCCTAGCTGGC | |||||
| Cypj122 | F: CCATCAGGCCACCATTCTG | (GA)7 | FAM | 221–223 | LC73800 |
| R: TGGTGTCTCCTTATTGTGATTGC | |||||
| Cypj140 | F: AGTTGGGTATCGAGGTGGC | (GA)13 | FAM | 174–176 | LC73801 |
| R: AGACTAAGCTATGGTAACTACATTCC | |||||
| Cypj147 | F: CCAGGACCTTAGCCCTGAC | (GA)6 | VIC | 375 | LC73802 |
| R: CCCTCTCAGATCTCCTACAAAGG | |||||
| Cypj179 | F: AGTTGGCAAGGATCTTATTGGC | (TA)6 | VIC | 247–249 | LC73803 |
| R: GCCCAGGCCCTTATTCAAAG | |||||
| Cypj180 | F: ACACCCATATTTGAGGATGGC | (TG)9 | FAM | 311 | LC73804 |
| R: AGCAGTTCCTAATGGCAAGG | |||||
| Cypj196 | F: AGCTCTCATACTGAGGGTTG | (CT)10 | VIC | 217–219 | LC73805 |
| R: TATGCACTTGGCACATTCG | |||||
| Cypj197 | F: ACCGATGAAATTTGGCAGAGG | (CT)8 | FAM | 258 | LC73806 |
| R: CACTCCCGCCATTAGAACC | |||||
| Cypj202 | F: TGCTAACATTTGCAACAAAGC | (AG)10 | FAM | 174–176 | LC73807 |
| R: TGCTTGGTGATGGAGGAAAC | |||||
| Cypj204 | F: TCCTCCAGCACTTTGTCGG | (AG)10 | VIC | 180 | LC73808 |
| R: TCCTACAAGCCTCCACTGC | |||||
| Cypj205 | F: ACTAGCATCGCTGAAAGTGC | (GA)10 | VIC | 277 | LC73809 |
| R: TGAGGAGAGACTCCATGAACG | |||||
| Cypj216 | F: AATCAATTCCCATTTAAAACTCTC | (CT)10 | VIC | 234 | LC73810 |
| R: ATTTAGGCCAAAACAGAGGA | |||||
| Cypj218 | F: ACCGGTGATGAAGGAAAATA | (TA)10 | VIC | 220–226 | LC73811 |
| R: TGATTTGAAGCCTAATATATAT | |||||
| Cypj224 | F: AAGAGGTTGGCTTTTGGATT | (TC)11 | VIC | 168–170 | LC73812 |
| R: CAACGATGAGTTCGTAAAGG | |||||
| Cypj233 | F: AAGCCAAAAGAGAAGCTTGA | (CT)10 | FAM | 214 | LC73813 |
| R: GAACTTGAACCCGAGAGAGA |
a
Annealing temperature for all reactions was 57°C.
b
Sequence of the fluorescent labels: FAM = 5′-CACGACGTTGTAAAACGAC-3′, VIC = 5′-TGTGGAATTGTGAGCGG-3′.