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. 2016 Feb 19;12(2):e1005870. doi: 10.1371/journal.pgen.1005870

Fig 5. EMSA analysis of CodY binding to select genes from the different clusters.

Fig 5

Binding of CodY to 11 regulatory regions of CodY regulated transcriptional units (hisZ, rsbV, glpF, argG, gadC, feoA, actA, gdhA, poxB, glnR and fliN) and a control probe (bglA). Primers used for amplification of DNA probes are described in S5 Table. Results are representative of at least 2 independent biological repeats. EMSA was performed with 10 mM BCAA for hisZ, rsbV, glpF, argG, gadC gdhA, poxB, glnR and fliN. For feoA and actA BCAAs were not added. Asterisks (*) point to unbound DNA probes.