Fig 3.

Lentivirus-mediated knock-down of AEG-1 gene in human retinoblastoma (RB) cell lines SO-RB50 and Y79; 3A: Human RB cell lines Y79 and SO-RB50 transfected with AEG-1-siRNA (KD groups) or with NC lentivirus and non-transfected cells were examined by fluorescent microscopy and light microscopy at the 3rd day after infection. More than 80% of RB cells expressed GFP. 3B: AEG-1-siRNA lentivirus transfected cultures had significantly lower levels of AEG-1 mRNA compared to levels in cultures transfected with NC lentivirus (*P<0.05). 3C: The protein expression level of AEG-1 decreased significantly in the KD groups compared to that in the NC groups. For comparison, the GAPDH protein did not vary significantly between the groups.