Fig. 2.
Oral administration of obeticholic acid is more effective in treating EAE than intraperitoneally (i.p.) injections. (A) Clinical EAE scores of mice administered 6-ECDCA or vehicle. Mice with established EAE were randomized into three groups (n = 9) and given daily doses of vehicle or 5 mg/kg of 6-ECDCA either i.p. or orally (oral). Each point represents mean ± SEM, *P < 0.05 by Mann–Whitney u test. (B) Seventy-two-hour proliferation assay of harvested splenocytes with increasing concentrations of MOG35–55. 3H-thymidine incorporation is measured in triplicate wells. Values are the mean + SEM of triplicates, *P < 0.05 by Student’s t test. (C) Cytokine production by harvested splenocytes with increasing concentrations of MOG35–55. Values are the mean + SEM of triplicates, *P < 0.05 by Student’s t test.