Fig. S3.

Nested probe set provides species-level identification of hedgehog Corynebacterium. Sample was hybridized with nine probes, each labeled with a different fluorophore, targeting cells at the level of kingdom, phylum, genus, and species. (Top) Two different probes targeting phylum Actinobacteria identify a consistent set of cells. Genus-level probes (Middle Left) identify these cells as the three genera Corynebacterium, Actinomyces, and Rothia and (Middle Right) are shown in the context of cells labeled with the universal probe Eub338 plus autofluorescence. (Bottom Left) A second genus-level probe validates the identity of Corynebacterium cells, and (Bottom Right) a species-level probe identifies them as Corynebacterium matruchotii.