Table S3.
Primers used in this study
| Primer na+G5:H94me | Sequence (5′–3′) |
| VPA0009_1F | AGGATAAACTCGAGCGGCATTAAGTTTGGGTAGTC |
| VPA0009_1R | AGTTAGGAATTCATGAAATCACTCCGATAATCTTG |
| VPA0009_2F | AGTTAGGAATTCGTAACTTGAAATTGATCAAGTTAAG |
| VPA0009_2R | AGTTAGAGATCTGCGCCTGCAGTTTTCGC |
| VPA0149_1F | GCTTATCGATACCGTCGACCCTCGATGAAAGCGCGCTGCAGTAC |
| VPA0149_1R | CGACTTGAACTGCGATGCCTGGCGCCCACAGATTCTAAATAAC |
| VPA0149_2F | CAGGCATCGCAGTTCAAGTCGCGGGTAACAATGAAGTGAACTCG |
| VPA0149_2R | CACTAGTGACGCGTACTCGAAGAAACGTTCACGGTTACGGC |
| VPA0710_1F | GCTTATCGATACCGTCGACCCTCGACTTCTTCTTCGCTGATCAAAC |
| VPA0710_1R | CGACTTGAACTGCGATGCCTGACTATCTAATTTGTCTACTCCGC |
| VPA0710_2F | CAGGCATCGCAGTTCAAGTCGTGGATTTAAAACAGAGCTCAGG |
| VPA0710_2R | CACTAGTGACGCGTACTCGACACTTGAGGTAATTAAAGCGGC |
| VPA0736_1F | GCTTATCGATACCGTCGACCCTCGAACGAACCCAGGAGAAGGTTG |
| VPA0736_1R | CGACTTGAACTGCGATGCCTGAAATGCTTCCTCTAATTGGAAAG |
| VPA0736_2F | CAGGCATCGCAGTTCAAGTCGTGTGATGGTGATTGATGACC |
| VPA0736_2R | CACTAGTGACGCGTACTCGACAAGCACCTCTCCACTTTG |
| VPA0763_1F | GCTTATCGATACCGTCGACCCTCGAGCAGCTGCAATCATCCAAATC |
| VPA0763_1R | CGACTTGAACTGCGATGCCTGGATAGGATTCCAAGAAATGACTTG |
| VPA0763_2F | CAGGCATCGCAGTTCAAGTCGTAAAAGTAGTCATAGTGGAAGATG |
| VPA0763_2R | CACTAGTGACGCGTACTCGACGGAACATAAAAGGATCTAAGC |
| VPA0920_1F | GCTTATCGATACCGTCGACCCTCGAATGGTCTAGTAGTTCATCACG |
| VPA0920_1R | CGACTTGAACTGCGATGCCTGGGACGATAAAAACTTGGCTG |
| VPA0920_2F | CAGGCATCGCAGTTCAAGTCGACTGTTACCGTTACTTGTTTC |
| VPA0920_2R | CACTAGTGACGCGTACTCGATCTGCCTGGTCACATGAACG |
| VPA0965_1F | GCTTATCGATACCGTCGACCCTCGAAAGCACGCACAGGCTTGC |
| VPA0965_1R | CGACTTGAACTGCGATGCCTGTAAAAGGACTCTTGAGAGAATAG |
| VPA0965_2F | CAGGCATCGCAGTTCAAGTCGCTCAAGATGTTTGGATTTTTACG |
| VPA0965_2R | CACTAGTGACGCGTACTCGAATAGGGATCAGAGCGCCAC |
| VPA1075_1F | GCTTATCGATACCGTCGACCCTCGACAGATACCAAGGAACACGAG |
| VPA1075_1R | CGACTTGAACTGCGATGCCTGCGTCCAAATTTGCCAATGAAG |
| VPA1075_2F | CAGGCATCGCAGTTCAAGTCGGAATAGTCTCTAATCGTGATTC |
| VPA1075_2R | CACTAGTGACGCGTACTCGACTGGCGTTGTTGGAAGATAAC |
| VPA1100_1F | GCTTATCGATACCGTCGACCCTCGATTTTCATGCGCCATGTGGTG |
| VPA1100_1R | CGACTTGAACTGCGATGCCTGAGTGGCTGTTCGCTCATTTC |
| VPA1100_2F | CAGGCATCGCAGTTCAAGTCGTTTCCAGCACCTCTAAAATTAC |
| VPA1100_2R | CACTAGTGACGCGTACTCGAGTTTAAACGCGCTGGAAACC |
| VPA1130_1F | GCTTATCGATACCGTCGACCCTCGAATACGCTGTTTGATATGGGATG |
| VPA1130_1R | CGACTTGAACTGCGATGCCTGTGCTTTTGTGAATCGTACTTGG |
| VPA1130_2F | CAGGCATCGCAGTTCAAGTCGCGCCCACTTATCTCGAAATG |
| VPA1130_2R | CACTAGTGACGCGTACTCGATATTACAGGGATTATTGTGGCG |
| VPA1277_1F | GCTTATCGATACCGTCGACCCTCGAAGATTTGATGCTGCCGGGC |
| VPA1277_1R | CGACTTGAACTGCGATGCCTGTTATGCGTTCCATGCATCCG |
| VPA1277_2F | CAGGCATCGCAGTTCAAGTCGCGGGCGAACAACGATAAAAG |
| VPA1277_2R | CACTAGTGACGCGTACTCGACGAGAGCTTGTGTTTTGGTG |
| VPA1433_1F | GCTTATCGATACCGTCGACCCTCGAAGTTTTCCCGGCTTGAGAATC |
| VPA1433_1R | CGACTTGAACTGCGATGCCTGGTTACAAAGTTTTAGTCGTGGAC |
| VPA1433_2F | CAGGCATCGCAGTTCAAGTCGCCAAAATTCGATGCAGAAAC |
| VPA1433_2R | CACTAGTGACGCGTACTCGATTGATCATGGACGAAGCAAC |
| VPA1515_1F | GCTTATCGATACCGTCGACCCTCGATGCCGGACTACATGGCTTTC |
| VPA1515_1R | CGACTTGAACTGCGATGCCTGGAAGTGTGAATCAGACAGATC |
| VPA1515_2F | CAGGCATCGCAGTTCAAGTCGCCTATCGCCTCCTCACAC |
| VPA1515_2R | CACTAGTGACGCGTACTCGATTGCGTCAACGGCAAGATTG |
| VPA1664_1F | GCTTATCGATACCGTCGACCCTCGATTTTGCTCAGCAAGCTCCATG |
| VPA1664_1R | CGACTTGAACTGCGATGCCTGGACTATGAACAAACTGGCATTC |
| VPA1664_2F | CAGGCATCGCAGTTCAAGTCGAAGGGAGGGTTTGAAAGAAAG |
| VPA1664_2R | CACTAGTGACGCGTACTCGACGATGCCGACCCGTTTGAAC |
| VP0362_1F | GCTTATCGATACCGTCGACCCTCGAATTATGTTGCCTAAACTCGATGG |
| VP0362_1R | CGACTTGAACTGCGATGCCTGGACTCTCTTCGAGTTTGTAGC |
| VP0362_2F | CAGGCATCGCAGTTCAAGTCGAAGGCAAACCTTGGCTTGCC |
| VP0362_2R | CACTAGTGACGCGTACTCGATTTGAAAGCAGTGGCCTGGC |
| VP0487_1F | GCTTATCGATACCGTCGACCCTCGATTCTTCAGACTGCGTGGCTC |
| VP0487_1R | CGACTTGAACTGCGATGCCTGTCACTACTCCATGGCACAAG |
| VP0487_2F | CAGGCATCGCAGTTCAAGTCGCATGAAGAAACTTGCACCCG |
| VP0487_2R | CACTAGTGACGCGTACTCGATGGTGACTTAGAAGACTAATTC |
| VP1069_1F | GCTTATCGATACCGTCGACCCTCGAATCGTACAACATGGTAAGAGAG |
| VP1069_1R | CGACTTGAACTGCGATGCCTGATCTGTAGACGAATCATTCCC |
| VP1069_2F | CAGGCATCGCAGTTCAAGTCGTATACAGGTAAAAAAGCAGTCC |
| VP1069_2R | CACTAGTGACGCGTACTCGACGGCATGACGAATACAAAATG |
| VP1201_1F | GCTTATCGATACCGTCGACCCTCGAGCAGCAGCATCCACTATTAC |
| VP1201_1R | CGACTTGAACTGCGATGCCTGCACCTACATCAAATGTTGTCC |
| VP1201_2F | CAGGCATCGCAGTTCAAGTCGTCTTTGTCCACTTTATATCGTTG |
| VP1201_2R | CACTAGTGACGCGTACTCGAATAGTGCGAAGCGAAACACAC |
| VP1211_1F | GCTTATCGATACCGTCGACCCTCGATTCAGGCTTGTCCATTTGG |
| VP1211_1R | CGACTTGAACTGCGATGCCTGAACGAATCACTAAAGACTCTTTTTC |
| VP1211_2F | CAGGCATCGCAGTTCAAGTCGTCAGTTGATACCCTAAGCC |
| VP1211_2R | CACTAGTGACGCGTACTCGAGACGCGATTGTGCTCGAC |
| VP1375_1F | GCTTATCGATACCGTCGACCCTCGATCGCCTTATGTGGTGCTATTC |
| VP1375_1R | CGACTTGAACTGCGATGCCTGTACATGTATTACCCAATATGCC |
| VP1375_2F | CAGGCATCGCAGTTCAAGTCGAGAACCGTTGAATAAATGGCTG |
| VP1375_2R | CACTAGTGACGCGTACTCGACGAAACAGAACGTGGCTGTG |
| VP1503_1F | GCTTATCGATACCGTCGACCCTCGATACCGTTTAAACGTTCTGAAAATTG |
| VP1503_1R | CGACTTGAACTGCGATGCCTGATCCCTCATCCTCAGAATCC |
| VP1503_2F | CAGGCATCGCAGTTCAAGTCGCGTAAACCAACGCCAGTTCC |
| VP1503_2R | CACTAGTGACGCGTACTCGATCATAAAGGTTGATTTACCACAAC |
| VP1712_1F | GCTTATCGATACCGTCGACCCTCGAGCTTCACCAACAAACAAGTCG |
| VP1712_1R | CGACTTGAACTGCGATGCCTGACATGGCAACGCGAGTGATG |
| VP1712_2F | CAGGCATCGCAGTTCAAGTCGGGTACATCCTTACCGTTAACC |
| VP1712_2R | CACTAGTGACGCGTACTCGACCAAATTGCCTGACTTCAATG |
| VP1735_1F | GCTTATCGATACCGTCGACCCTCGAGCGTTCACTGCCGTAATTTTG |
| VP1735_1R | CGACTTGAACTGCGATGCCTGGAATGTTTTTGATGGCGACG |
| VP1735_2F | CAGGCATCGCAGTTCAAGTCGCGAAAAAAGACGACTAAAAAGG |
| VP1735_2R | CACTAGTGACGCGTACTCGACAAGAATCCATAGCCCAGCG |
| VP1908_1F | GCTTATCGATACCGTCGACCCTCGACGTCGCGATTTTCTTACTGC |
| VP1908_1R | CGACTTGAACTGCGATGCCTGTGTGGTGATCGTCGATGATC |
| VP1908_2F | CAGGCATCGCAGTTCAAGTCGCCTTCCCTCCATGAAACGC |
| VP1908_2R | CACTAGTGACGCGTACTCGAGCGTTTACGTATACAGGCGG |
| VP1968_1F | GCTTATCGATACCGTCGACCCTCGAGGTATGAGATTTTGCCCTTG |
| VP1968_1R | CGACTTGAACTGCGATGCCTGGTTCATTCACCTGCGTATTTTG |
| VP1968_2F | CAGGCATCGCAGTTCAAGTCGTTGGCATTAAAACTGCTTAACTG |
| VP1968_2R | CACTAGTGACGCGTACTCGAGTGAATAGCTCAAGGGATTTG |
| VP1984_1F | GCTTATCGATACCGTCGACCCTCGACATGCTGCCGCGCATGG |
| VP1984_1R | CGACTTGAACTGCGATGCCTGGCACCCGGCTCCAAGC |
| VP1984_2F | CAGGCATCGCAGTTCAAGTCGTTGCGATGCCGATTCCGACT |
| VP1984_2R | CACTAGTGACGCGTACTCGATATTGTTGAGCAGATGGTGG |
| VP2010_1F | GCTTATCGATACCGTCGACCCTCGAATTGCAATGCACAGCTCATCC |
| VP2010_1R | CGACTTGAACTGCGATGCCTGCTAATCGGTATTTAGGAGCAG |
| VP2010_2F | CAGGCATCGCAGTTCAAGTCGTCCTTGCAGATATTCTTGTTCAG |
| VP2010_2R | CACTAGTGACGCGTACTCGAATTGGCGCTTGGTTTTCAATAC |
| VP2478_1F | GCTTATCGATACCGTCGACCCTCGAACGGTTTGTCTGGTCGCG |
| VP2478_1R | CGACTTGAACTGCGATGCCTG GGATAAGATTACAACTCGTAAAG |
| VP2478_2F | CAGGCATCGCAGTTCAAGTCG ACCTCTCATAAAATACAAGCCTC |
| VP2478_2R | CACTAGTGACGCGTACTCGA GCGCTGAAAGCGTTAATTAATC |
| VP2567_1F | GCTTATCGATACCGTCGACCCTCGAGTCAATTGTTGGTCATAATTGAG |
| VP2567_1R | CGACTTGAACTGCGATGCCTGGTTATCTAATTACATCGGAGTC |
| VP2567_2F | CAGGCATCGCAGTTCAAGTCGGCATTCGGACTAAACTTCATAC |
| VP2567_2R | CACTAGTGACGCGTACTCGAAGTCTGTGGCAGAGATGAAAG |
| VP2859_1F | GCTTATCGATACCGTCGACCCTCGAGAAACGCTGAAGCGTCTGC |
| VP2859_1R | CGACTTGAACTGCGATGCCTGTAATCCTCCTCAAGCAGCATATA |
| VP2859_2F | CAGGCATCGCAGTTCAAGTCGCTCTACAAGATTTCAGTTGATAATG |
| VP2859_2R | CACTAGTGACGCGTACTCGAGTCTTTGAACTTTTCGAAGTG |
| VP2874_1F | GcttatcgataccgtcgaccctcgaCGGTTATTCCTGAAGCCGC |
| VP2874_1R | CGACTTGAACTGCGATGCCTGCTTAGGTAAGTTGAACGCATG |
| VP2874_2F | CAGGCATCGCAGTTCAAGTCGCCGATATCCTTTCTAGCTTTTC |
| VP2874_2R | CACTAGTGACGCGTACTCGAGCGCACGCTGTTTATTGTTG |
| VPA0919_1F | GCTTATCGATACCGTCGACCCTCGACAATTGGCGGAGGCAAGTAAAGA |
| VPA0919_1R | CGACTTGAACTGCGATGCCTGCTGCTTGTTCCAGGCTTAC |
| VPA0919_2F | CAGGCATCGCAGTTCAAGTCGACTGTAGATACCCACGTACTTC |
| VPA0919_2R | CACTAGTGACGCGTACTCGAACTCATCAACCCAACGACG |
| VPA 0477_1F | GCTTATCGATACCGTCGACCCTCGACACGGCGGCAATTATCATCC |
| VPA 0477_1R | CGACTTGAACTGCGATGCCTGGCGTGGTGTTCTTCGTTCTT |
| VPA 0477_2F | CAGGCATCGCAGTTCAAGTCGGAACTCGAGCAACCAAGCC |
| VPA 0477_2R | CACTAGTGACGCGTACTCGAACAGTATCGAGCACCTGCTG |
| VPA0920_pMMB_F | AGCTCGGTACCCGGGGATCCTCTAGTTGATTAAACAGTTTTTGCTCGG |
| VPA0920_pMMB_R | TCTCATCCGCCAAAACAGCCAAGCTTCAACGAGAAGCAGTGTCTGTT |
| VPA0919_pMMB_F | GGTACCCGGGGATCCTCTAGTAAGGAGGTAGGATAATAGTGAAACAGACACTGCTTC |
| VPA0919_pMMB_R | TCTCATCCGCCAAAACAGCCAAGCTTTATGCTTTCATCTTGTAGCCTA |
| vpa0477_RBS_pMMB_F | GGTACCCGGGGATCCTCTAGTAAGGAGGTAGGATAATAATGAAAAAGTTATTCCTGTTGG |
| vpa0477_pMMB_R | TCCGCCAAAACAGCCAAGCTTTAACTTTCTTTGTAGTGCTCTA |
| VPA0920_pMMB_His_R | TCTCATCCGCCAAAACAGCCAAGCTTCAATGGTGGTGGTGATGATGTACGAGAAGCAGTGTCTGTT |
| VPA0919_pMMB_His_R | TCTCATCCGCCAAAACAGCCAAGCTTTAATGGTGGTGGTGATGATGATGTGCTTTCATCTTGTAGCCTA |
| VPA0920H286A_1R | AGCCGTTAAGATCTGTAAGAC |
| VPA0920H286A_2F | GTCTTACAGATCTTAACGCTGAGCTTCGAACGCC |
| VPA0919D51A_1R | AGCTAGGATGACAAGATCCG |
| VPA0919D51A_2F | CGGATCTTGTCATCCTAGCTCGCCAGCTACCTG |
| VPA0477_RT_F | GCGACATGGACAGCGGCAA |
| VPA0477_RT_R | AATCGGTCAAGTCGCGTTGC |
| secY_RT_F | GGCATCATGCCGTATATTTCG |
| secY_RT_R | GGCAATCCAGCAACAATACC |
| 920SD_GST_FBamHI | AACGGATCCCTACCAGAACGCATAGATACG |
| 920SD_GST_RXhoI | AACCTCGAGGTGATCTTCTACATCCCAAC |
| 920L82A_1R | CAGCGCAGCGAATACATGTC |
| 920L82A_2F | GACATGTATTCGCTGCGCTG |
| 920P125A_1R | CACGCAGGTGGCGGTACTTAC |
| 920P125A_2F | GTAAGTACCGCCACCTGCGTG |
| 920L95A_1R | CCTGCAATTACTGAACCTCTAG |
| 920L95A_2F | CTAGAGGTTCAGTAATTGCAGG |