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. 2016 Jan 21;113(6):E772–E781. doi: 10.1073/pnas.1514530113

Fig. S2.

Fig. S2.

Single-molecule trajectory analysis of lipid-loaded CD1d molecules at longer observation times. Trajectories longer than 100 frames have been selected for the analysis. (A, D, and G) Representative MSD (black dots) plots of the first 250 ms (25 points) of individual trajectories of (A) α-GalCer–loaded, (D) unpulsed, and (G) Gal-GalCer–loaded hCD1d molecules. Individual MSD curves were fitted with the function MSD = 4Dtα (red line), where the exponent-α accounts for the degree of anomalous diffusion. (B, E, and H) Diffusion coefficients and (C, F, and I) α-values obtained from the fitting belonging to (B and C) α-GalCer–loaded, (E and F) unpulsed, and (H and I) Gal-GalCer–loaded hCD1d molecules. Significant reductions in the α-values were obtained on disruption of actin cytoskeleton interactions for the cases of exogenous lipid–loaded hCD1d molecules (α-GalCer and Gal-GalCer), whereas no changes in the α-values were observed for the unpulsed case. Data (same as Fig. 1) from typically 200 trajectories per condition from at least 25 cells over eight different experiments. ns, Not significant (P > 0.05; one-way ANOVA test). *P < 0.05 (one-way ANOVA test); **P < 0.0001 (one-way ANOVA test).