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. 2016 Jan 7;113(6):E735–E743. doi: 10.1073/pnas.1523838113

Fig. 1.

Fig. 1.

Illumina RNA-seq analysis of Met and Hairy RNAi-depleted A. aegypti mosquito fat body. (A) WBs showing the presence of Met and Hairy proteins in iLuc, iMet, and iHairy samples. WBs also provided proof of the efficiency of dsRNA-mediated knockdowns. Anti–β-actin antibody was used as a loading control. (B) Venn diagram of Met and Hairy RNAi fat-body transcriptome analysis showing that 79% of iHairy up-regulated genes are also up-regulated by iMet. iMET↑, more than twofold up-regulation by dsRNA depletion of Met (AKA, Met-repressed); iHairy↑, more than twofold up-regulation by dsRNA depletion of Hairy (AKA, Hairy-repressed); iMet/iHairy↑↑, more than twofold up-regulation by dsRNA depletion of Met and Hairy (AKA, Met/Hairy-repressed). All comparisons were made with the control iLuc fat-body transcriptome.