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. 2016 Jan 25;113(6):1552–1557. doi: 10.1073/pnas.1519803113

Fig. 6.

Fig. 6.

UMOD ZP-N homodimerization is essential for filament formation. (A) UMOD ZP-N/ZP-N interface. Molecule A is in a solvent-accessible surface (Left: hydrophobic, red; hydrophilic, white) or depicted in a cartoon (Right, blue) representation; molecule B is depicted in a cartoon representation (green). Interface residues and disulfides are depicted in a ball-and-stick representation and are colored green (hydrophilic/charged), cyan (hydrophobic), gray (N396 glycan-interacting residues), and magenta (I421 and L333). (B) Immunofluorescence analysis of MDCK cells stably expressing full-length, HA-tagged, WT UMOD. (Scale bar, 50 μm.) (C) Immunogold labeling of full-length, HA-tagged, WT UMOD filaments produced in MDCK cells, with the same anti-HA primary Ab used in B. Two different areas are shown. (Scale bars, 0.2 μm.) (D and E) Immunofluorescence analysis of MDCK cells stably expressing full-length, HA-tagged UMOD dimerization interface mutants L333K and I421K. (Scale bars, 50 μm.)