Fig. 4.

Histograms of unfolding trajectories at 15 and 45 pN using a 2D map with the global order parameter (χ) and the distance between Leu24 and Glu52 side chains, which shows whether the RT loop interacts with the protein core (Fig. 2A). The profile offers an alternative view of the one in terms of χ and ${\chi }_{RTL}$ (Fig. 3).