Fig. S3.

Unfolding rates of titin I27 domain as a function of guanidinium chloride concentration for the WT and various mutants (8). Dashed lines show single-exponential fits $k_u=k^{{\text{H}}_2\text{O}}\exp \left(m^{‡}\left[C\right]\right)$, and solid lines show double exponential fits $k_u=k_L^{{\text{H}}_2\text{O}}\exp \left(m_L\left[C\right]\right)+k_H^{{\text{H}}_2\text{O}}\exp \left(m_H\left[C\right]\right)$. The extracted values of the parameters are in Table S2.