Fig. 3.

PP2A^B56 controls APC/C recruitment and activation of Ube2S. (A) PP2A^B56 restores cross-linking of Ube2S to Apc11 on phosphatase-inhibited APC/C purified and treated with BMB as described above. (B) PP2A^B56 restores the activity of phosphatase-inhibited APC/C toward Ube2S. APC/C was purified from prometaphase extracts treated with okadaic acid, as indicated, and its activity to promote Ube2S-dependent ubiquitin chain elongation on Ub-cyclin A was measured by Western blot analysis. Where indicated, purified PP2A^B56α was added to the reactions.