Fig. S4.

Kinetochore function regulates the interaction of Cdc20 with Ube2S and PP2A^B56. (A) Depletion of Knl1 from HeLa cells displaces Cdc20 from kinetochores. HeLa cells were transfected with siRNA against Knl1, and localization of Cdc20 was determined in prometaphase by immunofluorescence microscopy against Cdc20 (green), Ndc80 (red), and DNA (blue). (B) Depletion of Ndc80 impairs binding of Cdc20 to PP2A^B56 and Ube2S. HeLa cells were depleted of Ndc80, synchronized in prometaphase with STLC and MG-132, and subjected to Cdc20 affinity purification and Western blot analysis.