Table S1.
Absolute numbers of leukocytes and B-lineage cells in bone marrow, spleen and Peyer’s patches of 8- to 12-wk-old mice carrying IgH alleles of different haplotypes: wta/wtb (129-C57BL/6 F1 mice), 3′PALΔa/wtb, hs3b‐4Δa/wtb, and 3′RRΔa/wtb
| Location | wta/wtb | IgMa/IgMb ratio | 3′PALΔa/wtb | IgMa/IgMb ratio | hs3b-4Δa/wtb | IgMa/IgMb ratio | 3′RRΔa/wtb | IgMa/IgMb ratio |
| Bone marrow | ||||||||
| Leukocytes | 83.7 ± 2.5 n = 11 | — | 79.8 ± 9.7 n = 9 | — | 66.4 ± 6.4 n = 4 | — | 71.1 ± 3.0 n = 11 | — |
| Total B cells (B220+) | 25.7 ± 1.7 n = 11 | — | 24.2 ± 3.7 n = 9 | — | 17.8 ± 1.8 n = 4 | — | 22.3 ± 0.9 n = 11 | — |
| IgMa immature B cells (B220int IgMa+) | 8.8 ± 0.2 n = 7 | 0.93 | 6.2 ± 0.7 n = 5 | 0.95 | 7.6 ± 0.4 n = 4 | 0.93 | 9.1 ± 0.5 n = 11 | 0.96 |
| IgMb immature B cells (B220int IgMb+) | 9.5 ± 0.3 n = 7 | 6.5 ± 0.8 n = 5 | 8.2 ± 0.8 n = 4 | 9.5 ± 0.6 n = 11 | ||||
| IgMa mature B cells (B220high IgMa+) | 8.3 ± 0.4 n = 7 | 0.94 | 8.1 ± 0.5 n = 5 | 0.93 | 4.6 ± 0.6 n = 4 | 0.43 | 7.9 ± 0.5 n = 11 | 0.57 |
| IgMb mature B cells (B220high IgMb+) | 8.8 ± 0.4 n = 7 | 8.7 ± 0.5 n = 5 | 10.6 ± 1.8 n = 4 | 13.9 ± 0.9 n = 11 | ||||
| Spleen | ||||||||
| Leukocytes | 149.2 ± 13.6 n = 19 | — | 172.3 ± 10.4 n = 14 | — | 179.7 ± 12.6 n = 9 | — | 156.6 ± 6.6 n = 16 | — |
| Total B cells (B220+ CD19+) | 44.9 ± 4.7 n = 11 | — | 51.3 ± 3.3 n = 9 | — | 50.7 ± 5.2 n = 4 | — | 54.8 ± 2.4 n = 11 | — |
| IgMa follicular B cells (CD23high CD21low IgMa+) | 18.0 ± 2.3 n = 7 | 1.09 | 16.1 ± 0.8 n = 5 | 0.89 | 6.1 ± 0.8 n = 4 | 0.21 | 8.5 ± 0.6 n = 7 | 0.25 |
| IgMb follicular B cells (CD23high CD21low IgMb+) | 16.5 ± 2.2 n = 7 | 18.1 ± 1.0 n = 5 | 29.1 ± 2.2 n = 4 | 34.1 ± 2.0 n = 7 | ||||
| IgMa marginal zone B cells (CD23low CD21high IgMa+) | 2.2 ± 0.2 n = 7 | 1.16 | 1.6 ± 0.2 n = 5 | 0.94 | 0.3 ± 0.04 n = 4 | 0.05 | 0.3 ± 0.03 n = 7 | 0.06 |
| IgMb marginal zone B cells (CD23low CD21high IgMb+) | 1.9 ± 0.1 n = 7 | 1.7 ± 0.2 n = 5 | 5.7 ± 0.4 n = 4 | 4.6 ± 0.3 n = 7 | ||||
| IgMa transitional B cells (AA4.1+ IgMa+) | 8.0 ± 0.6 n = 7 | 1.13 | 5.2 ± 0.8 n = 5 | 0.93 | 4.2 ± 0.5 n = 4 | 0.44 | 3.4 ± 0.5 n = 7 | 0.44 |
| IgMb transitional B cells (AA4.1+ IgMb+) | 7.1 ± 0.6 n = 7 | 5.6 ± 0.8 n = 5 | 9.5 ± 1.0 n = 4 | 7.7 ± 1.1 n = 7 | ||||
| Peyer's patches | ||||||||
| Leukocytes | 15.0 ± 1.3 n = 11 | — | 20.6 ± 3.7 n = 9 | — | 14.2 ± 3.3 n = 4 | — | 11.0 ± 2.4 n = 7 | — |
| Total B cells (B220+) | 9.8 ± 0.9 n = 11 | — | 14.6 ± 2.8 n = 9 | — | 10.9 ± 2.4 n = 4 | — | 8.3 ± 1.9 n = 7 | — |
| IgMa B cells | 3.9 ± 0.3 n = 11 | 1.11 | 5.2 ± 0.7 n = 9 | 1.21 | 2.2 ± 0.5 n = 4 | 0.30 | 2.0 ± 0.5 n = 7 | 0.37 |
| IgMb B cells | 3.5 ± 0.3 n = 11 | 4.3 ± 0.6 n = 9 | 7.2 ± 1.6 n = 4 | 5.4 ± 1.3 n = 7 |
Means ± SEM are reported; number of animals is reported; within a given B-cell subset expressing a- or b- allele, the a/b ratio is reported.