Real-time qRT-PCR |
|
|
Cd79a
|
Mm00432423_m1 |
Applied Biosystems |
Gapdh
|
Mm99999915_g1 |
Applied Biosystems |
IgH primary transcripts |
|
|
IgHWT for |
5′TTCTGAGCATTGCAGACTAATCTTG3′ |
(31) |
IgHWT rev |
5′CCTAGACAGTTTATTTCCCAACTTCTC3′ |
(31) |
IgHWT probe |
5′CCCTGAGGGAGCCG3′ |
(31) |
Germ-line transcripts |
|
|
Iμ-Cμ for |
5′ACCTGGGAATGTATGGTTGTGGCTT3′ |
(46) |
Iμ-Cμ rev |
5′TCTGAACCTTCAAGGATGCTCTTG3′ |
(46) |
Iγ3-Cγ3 for |
5′AACTACTGCTACCACCACCACCAG3′ |
(46) |
Iγ3-Cγ3 rev |
5′ACCAAGGGATAGACAGATGGGG3′ |
(46) |
Iγ1-Cγ1 for |
5′GGCCCTTCCAGATCTTTGAG3′ |
(46) |
Iγ1-Cγ1 rev |
5′ATGGAGTTAGTTTGGGCAGCA3′ |
(46) |
Iγ2b-Cγ2b for |
5′CCAACCAGGAAGAGTCCAGAG3′ |
(48) |
Iγ2b-Cγ2b rev |
5′ACAGGGATCCAGAGTTCCAAGT3′ |
(48) |
Iγ2a-Cγ2a for |
5′GCTGATGTACCTACCGAGAGA3′ |
(46) |
Iγ2a-Cγ2a rev |
5′GCTGGGCCAGGTGCTCGAGGTT3′ |
(46) |
Iα-Cα for |
5′CTACCATAGGGAAGATAGCCT3′ |
(47) |
Iα-Cα rev |
5′TAATCGTGAATCAGGCAG3′ |
(47) |
LNC-RNA for CSR transcripts |
|
|
Lnc-RNA-CSR for |
5′-CACATTTGATTGCCTCTCCACTTG-3′ |
(38) |
Lnc-RNA-CSR rev |
5′-AAAACATCCCTCCAACCAGTCAC-3′ |
(38) |
SHM |
|
|
IgH locus |
|
|
VHJ558 family for |
5′GCGAAGCTTARGCCTGGGRCTTCAGTGAAG3′ |
(9) |
5′MAREμ rev |
5′CAGCAACTACCCTTTTGAGACCGA3′ |
Present study |
Igκ locus |
|
|
Igκ for |
5′GGCTGCAGSTTCAGTGGCAGTGGRTCWGGRAC3′ |
(31) |
Igκ rev |
5′AGCGAATTCAACTTAGGAGACAAAAGAGAGAAC3′ |
(31) |