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. 2016 Feb 1;113(6):1618–1623. doi: 10.1073/pnas.1514090113

Table S3.

Primers for real-time qRT-PCR and SHM used in this study

qRT-PCR or SHM Primer/probe Source
Real-time qRT-PCR
Cd79a Mm00432423_m1 Applied Biosystems
Gapdh Mm99999915_g1 Applied Biosystems
 IgH primary transcripts
  IgHWT for 5TTCTGAGCATTGCAGACTAATCTTG3 (31)
  IgHWT rev 5CCTAGACAGTTTATTTCCCAACTTCTC3 (31)
  IgHWT probe 5CCCTGAGGGAGCCG3 (31)
 Germ-line transcripts
  Iμ-Cμ for 5ACCTGGGAATGTATGGTTGTGGCTT3 (46)
  Iμ-Cμ rev 5TCTGAACCTTCAAGGATGCTCTTG3 (46)
  Iγ3-Cγ3 for 5AACTACTGCTACCACCACCACCAG3 (46)
  Iγ3-Cγ3 rev 5ACCAAGGGATAGACAGATGGGG3 (46)
  Iγ1-Cγ1 for 5GGCCCTTCCAGATCTTTGAG3 (46)
  Iγ1-Cγ1 rev 5ATGGAGTTAGTTTGGGCAGCA3 (46)
  Iγ2b-Cγ2b for 5CCAACCAGGAAGAGTCCAGAG3 (48)
  Iγ2b-Cγ2b rev 5ACAGGGATCCAGAGTTCCAAGT3 (48)
  Iγ2a-Cγ2a for 5GCTGATGTACCTACCGAGAGA3 (46)
  Iγ2a-Cγ2a rev 5GCTGGGCCAGGTGCTCGAGGTT3 (46)
  Iα-Cα for 5CTACCATAGGGAAGATAGCCT3 (47)
  Iα-Cα rev 5TAATCGTGAATCAGGCAG3 (47)
 LNC-RNA for CSR transcripts
  Lnc-RNA-CSR for 5-CACATTTGATTGCCTCTCCACTTG-3 (38)
  Lnc-RNA-CSR rev 5-AAAACATCCCTCCAACCAGTCAC-3 (38)
SHM
 IgH locus
  VHJ558 family for 5GCGAAGCTTARGCCTGGGRCTTCAGTGAAG3 (9)
  5′MAR rev 5CAGCAACTACCCTTTTGAGACCGA3 Present study
 Igκ locus
  Igκ for 5GGCTGCAGSTTCAGTGGCAGTGGRTCWGGRAC3 (31)
  Igκ rev 5AGCGAATTCAACTTAGGAGACAAAAGAGAGAAC3 (31)

Some oligonucleotides have been previously described in refs. 9, 31, 38, and 4648.