Table 1.
Clinical Characteristics of IBS Patients and Healthy Controls
| Overall samples | HM450 methylation array | Targeted bisulphite sequencing | |||||||
|---|---|---|---|---|---|---|---|---|---|
| IBS (N=27) | HC (N=23) | P | IBS (N=12) | HC (N=12) | P | IBS (N=25) | HC (N=21) | P | |
| Age [Mean (SD)] | 40.22 (9.12) | 36.48 (8.63) | 0.14 | 39.83 (3.43) | 39.83 (3.79) | 1 | 40.20 (9.49) | 36.52 (9.00) | 0.18 |
| Female % | 59.26 | 60.86 | 1 | 58.33 | 58.33 | 1 | 60.00 | 57.14 | 1 |
| Bowel habit [n (%)] IBS-C | 10 (37.03) | - | - | 3 (25) | - | - | 9 (36) | - | - |
| IBS-D | 8 (29.63) | - | - | 3 (25) | - | - | 8 (32) | - | - |
| IBS-M | 9 (33.33) | - | - | 6 (50) | - | - | 8 (32) | - | - |
| IBS usual severity (1–5) | 3.19 (0.49) | - | - | 3.18 (0.60) | - | - | 3.25 (0.44) | - | - |
| ETI-SR total score [Mean (SD)] | 6.78 (6.7) | 4.22 (3.98) | 0.1 | 8.33 (7.79) | 2.5 (1.57) | 0.03 | 6.72 (6.97) | 4.52 (4.11) | 0.19 |
| ETI-SR emotional abuse score [Mean (SD)] | 1.74 (1.93) | 1(1.76) | 0.16 | 2.08 (1.97) | 0.25 (0.45) | 0.01 | 1.6 (1.94) | 1.09 (1.81) | 0.37 |
| HAD-Anxiety [Mean (SD)] | 5.96 (5.63) | 2.52 (2.37) | 0.01 | 5.00(3.74) | 2.33 (1.87) | 0.04 | 6.04 (5.85) | 2.48 (2.46) | 0.01 |
| HAD-Depression [Mean (SD)] | 2.59 (3.39) | 0.74 (1.01) | 0.01 | 2.08 (1.97) | 0.75 (1.29) | 0.06 | 2.68 (3.48) | 0.71 (1.01) | 0.01 |
| PSS [Mean (SD)] | 12 (8.73) | 8.65 (7.29) | 0.15 | 10.33 (6.84) | 10.17 (7.84) | 0.96 | 12.40 (8.84) | 8.19 (7.19) | 0.08 |
| Psychiatric history -Current* [n (%)] | 1 (3.7%) | None | - | None | None | - | 1 (4%) | None | - |
| Psychiatric history - Past** [n (%)] | 3 (11.11%) | 3 (13.04%) | - | 2 (16.67%) | 1 (8.33%) | - | 3 (12.00%) | 3 (14.28%) | - |
| Race/ethnicity [n (%)] Caucasian | 7 (25.92) | 11 (47.83) | - | 3 (25.00) | 7 (58.33) | - | 7 (28.00) | 10 (47.62) | - |
| African American | 6 (22.22) | 4 (17.39) | - | 2 (16.67) | 3 (25.00) | - | 5 (20.00) | 4 (19.05) | - |
| Hispanic | 1 (03.70) | 1 (4.35) | - | 0 | 0 | - | 0 | 1 (4.76) | - |
| Asian | 4 (14.81) | 3 (13.04) | - | 2 (16.67) | 0 | - | 5 (20.00) | 3 (14.29) | - |
| Other (including multiracial) | 9 (33.33) | 4 (17.39) | - | 5 (41.67) | 2 (16.67) | - | 8 (32.00) | 3 (14.29) | - |
Abbreviations: IBS, irritable bowel syndrome; IBS-C, IBS with constipation; IBS-D, IBS with diarrhea; IBS-M, IBS with mixed bowel habit pattern; HC, healthy controls; SD, standard deviation; ETI-SR, emotional trauma inventory- self report; HAD, Hospital anxiety and depression scale; PSS, perceived stress score;
*
Current psychiatric history included 1 depression;
**
Past psychiatric history included 3 substance dependence or abuse (2 IBS, 1 control), 3 depression (1 IBS, 2 controls), 1 manic episode (1 IBS).