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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Hippocampus. 2015 Oct 10;26(3):341–361. doi: 10.1002/hipo.22526

Figure 1. Measurement and quantification of the anatomical predictors of slice position in the longitudinal axis.

Figure 1

A) Representative images of hippocampal slices obtained from sectioning an entire hippocampus from the ventral to dorsal (V–D) end. Index-slice represents the slice where the morphology of DG granule cell layer changed from U-Shape to V-shape. B) Scheme for measurement of anatomical markers associated with the hippocampal formation depicted using representative slices close to ventral (left, slice # 13) and dorsal end (right, slice # 32): CA1, transverse length (CA1_TL, dashed brown line) and radial length of stratum lacunosum moleculare (CA1_SLM, solid brown line); CA3, transverse length (CA3_TL, dashed blue line) and radial length (CA3_RL, solid blue line); DG, transverse length (DG_TL, dashed red line) and tip-to-tip distance (DG_TTD, solid red line). C-E) Transverse (circles) and radial (triangles) measurements from CA1 (C), CA3 (D) and DG (E) are plotted against the slice number (V–D) for one representative hippocampus. The ratios of the transverse-to-radial dimensions (black diamonds) of CA1 (C), CA3 (D), and DG (E) are plotted against the slice number. There was a significant linear correlation (tested using Pearson correlation coefficient) between the three ratios and slice position (n=27 slices; CA1_ratio: r= 0.98, p<0.01; CA3_ratio: r=0.88, p<0.01; DG_ratio: r=0.97, p<0.01) indicating that these ratios are very good predictors of slice position in the longitudinal axis. Black lines are regression lines.