Table 1. PCR primers and RFLP conditions for G6PD variants common in Southwest Sumba Regency and PCR primers for detecting SAO, HbE and α thalassemia.

Variant	Primer	Primer Sequence (5’ → 3’)	Expected PCR Product (bp)	RE	Expected Result (bp)		References
					Deficient	Normal
Vanua Lava	VL-9F	CAG CCT GGG GCA GTG TCT GTG CT	366	EcoNI	366	346	Our design
	VL-9R	GCG GTT GGC CTG TGA CCC CTG GTG				20
Viangchan	VC-9F	TGG CTT TCT CTC AGG TCT AG	126	XbaI	106	126	Nuchprayoon et al, 2002
	VC-9R	GTC GTC CAG GTA CCC TTT GGG G			20
Chatham	CT-9F	CAA GGA GCC CAT TCT CTC CCT T	208	BstXI	100	130	Gandomani et al, 2011
	CT-9R	TTC TCC ACA TAG AGG AGG ACG GCT GCC AAA GT			78	78
					30
Kaiping	KP-9F	ACG TGA AGC TCC CTG ACG C	227	MnlI	206	227	Laosombat et al, 2006
	KP-9R	GTG CAG CAG TGG GGT GAA CAT A			21
SAO	OVF 1098	GGG CCC AGA TGA CCC TCT TGC	175 (148 for SAO)	-	-	-	Jarolim et al, 1991
	OVR 1272	GCC GAA GGT GAT GGC GGG TG
HbE	Com C	ACC TCA CCC TGT GGA GCC AC	293	MnlI	122	106	Pramoodjago et al, 1999
	TLR 62320	CTA TTG GTC TCC TTA AAC CTG TCT TGT AAC CTT GCT A			106	60
alpha Thalassemia (Multiplex PCR—2 gene deletion)	SEA-alpha F	CTC TGT GTT CTC AGT ATT GGA GGG AAG GAG	1110 660	-	-	-	Liu et al, 2000
	SEA-alpha R	ATA TAT GGG TCT GGA AGT GTA ACC CTC CCA
	alpha R	TGA AGA GCC TGC AGG ACC AGG TCA GTG ACC G
	FILL-alpha F	AAG AGA ATA AAC CAC CCA ATT TTT AAA TGG GCA	550	-	-	-
	FILL-alpha R	GAG ATA ATA ACC TTT ATC TGC CAC ATG TAG CAA
	THAI-alpha F	CAC GAG TAA AAC ATC AAG TAC ACT CCA GCC	411	-	-	-
	THAI-alpha R	TGG ATC TGC ACC TCT GGG TAG GTT CTG TAC C
α Thalassemia (Multiplex PCR—1 gene deletion)	2/3P	TGT TGG CAC ATT CCG GGA CAG	1940 (normal)	-	-	-	Setianingsih et al, 2003
	XY1	GCG CCG AGC CTG GCC AAA CCA TCA CTT TTC	2220 (-3.7 kb deletion)
	3R1	TGC ATC CTC AAA GCA CTC TAG GGT CCA GCG T	1673 (-4.2 kb deletion)
	SA3P	TAA GCT AGA GCA TTG GTG GTC ATG C
	XYHA	GAA GTA CGT CCG ACC AGC TTA GCC A

RE is restriction enzyme; bp is base pair.