Table 1. Sequences of primers and PCR reaction parameters used in real-time RT-PCR.
Target gene | Forward | Reverse | AT (°C) |
---|---|---|---|
LPL | AACCTTTGTGGTGATCCATGGA | CGAAATCCGCATCATCAGGA | 60 |
aP2 | ATGTGTGATGCCTTTGTGGG | CCCAGTTTGAAGGAAATCTC | 55 |
Lipin1 | TCACTACCCAGTACCAGGGC | TGAGTCCAATCCTTTCCCAG | 55 |
DGAT1 | TATTACTTCATCTTTGCTCC | AAAGTAGGTGACAGACTCAG | 45 |
PEPCK | TGGGTGATGACATTGCCTGG | ACCTTGCCCTTATGCTCTGCAG | 60 |
ATGL | CATTTTAGCTCCAAGGATGA | TGGTTCAGTAGGCCATTCCT | 55 |
PDK2 | GGGGTGTCCCCTTGAGGAAGAT | TTCTTGGGCTCTGTGCTGGG | 55 |
mCPT1 | CGGAAGCACACCAGGCAGTA | GCAGCTTCAGGGTTTGTCGGAATA | 60 |
LCAD | AGCTCCCACAGGAAAGGCTT | CTCGAGCATCCACGTAGGCT | 55 |
GAPDH | TGAACGGGAAGCTCACTGG | TCCACCACCCTGTTGCTGTA | 60 |