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. 2016 Feb 19;11(2):e0148141. doi: 10.1371/journal.pone.0148141

Table 1. Sequences of primers and PCR reaction parameters used in real-time RT-PCR.

Target gene Forward Reverse AT (°C)
LPL AACCTTTGTGGTGATCCATGGA CGAAATCCGCATCATCAGGA 60
aP2 ATGTGTGATGCCTTTGTGGG CCCAGTTTGAAGGAAATCTC 55
Lipin1 TCACTACCCAGTACCAGGGC TGAGTCCAATCCTTTCCCAG 55
DGAT1 TATTACTTCATCTTTGCTCC AAAGTAGGTGACAGACTCAG 45
PEPCK TGGGTGATGACATTGCCTGG ACCTTGCCCTTATGCTCTGCAG 60
ATGL CATTTTAGCTCCAAGGATGA TGGTTCAGTAGGCCATTCCT 55
PDK2 GGGGTGTCCCCTTGAGGAAGAT TTCTTGGGCTCTGTGCTGGG 55
mCPT1 CGGAAGCACACCAGGCAGTA GCAGCTTCAGGGTTTGTCGGAATA 60
LCAD AGCTCCCACAGGAAAGGCTT CTCGAGCATCCACGTAGGCT 55
GAPDH TGAACGGGAAGCTCACTGG TCCACCACCCTGTTGCTGTA 60