Fig 2. GeneXpert Detection of mutations in rpoB RRDR region.
Results from twenty-five Rif resistant samples are shown. The results produced by each sample are indicated by a single vertical line on which the CT value of each of the five rpoB-specific molecular beacons [probes A to E] is plotted. Twenty-three samples were correctly identified as Rif resistant. Two samples [731GE, 919GE] were missed by GeneXpert but showed mutations in RRDR region on rpoB gene Sequencing [533CGG, 517AAG and 519CAC respectively] [Genbank Accession Numbers KP658669-658720]. Two strains with 531TTG mutation were detected owing to difference between the CT value being >4 and not due to loss of probe.