Fig 5. GH and PRL protein expression in a series of wt mouse and rat brains.

Protein expression of GH and PRL in mouse (A) and rat (B) brains, respectively. 16 animals are represented for FVB/NCr mice and 8 for Long-Evans rat. The (+) lane represents 4 ng of pituitary extract as positive control. “M” represents molecular weight marker. Panels C and D represent the GH and PRL expression in dissected sub-region of mouse brain. (C) Mouse brains were cut horizontally and sagittally as depicted in the accompanying diagram to generate 6 types of samples: Upper left (T1), upper right (T2), lower left (B1), lower right (B2), median eminence (ME), and brain stem (BS). (D) Diagram represents the ventral aspect of the brain showing ME and BS. Expression of GH and PRL proteins are represented for each sub-region, as well as loading controls probed for beta-actin.