Table 1. Gene targets and nucleotide sequences of primers used to amplify JEV gene segments.

Gene	Primer	Sequence	Product size	Refs.
FLAVI NS5	FLAVI-1	5’-AATGTACGCTGATGACACAGCTGGCTGGGACAC- 3’	863 bp	Ayers et al., 2006
	FLAVI-2	5’-TCCAGACCTTCAGCATGTCTTCTGTTGTCATCCA–3’
JEV-prM	JEV-prMf	5’-CGTTCTTCAAGTTTACAGCATTAGC-3’	675 bp	Wang et al., 2007
	JEV-prMr	5’-CCYRTGTTYCTGCCAAGCATCCAMCC-3’
JEV-E	JEV-Ef	5’-TGYTGGTCGCTCCGGCTTA-3’	1582 bp	Wang et al., 2007
	JEV-Er	5’-AAGATGCCACTTCCACAYCTC-3’