FIG. 3.

Statistical evaluation of the device performance based on both simulation and experiment derived data. (a) Effect of the channel height on the number of cells trapped at a single trap position showing 16 μm channel height is optimum for single cell trapping. (b) Monte Carlo simulation estimates of the trapping efficiency based on the trap positions (centered or optimized) and number of traps/channel (10, 25, and 50 traps/ch). (c) Monte Carlo simulation (continuous lines) and experiment (scattered points) trends for 6 combinations of the trap numbers and positions in microfluidic channels, indicating the highest efficiency ∼70% on the optimized pattern, 50 trap/ch device type.