FIG. 4.
On-chip cell culture evaluation. (a) Time dependent MIA PaCa-2 (initial observed cell # = 73) cell proliferation rate in the cell trapping device calculated by extracting and binning the cell division times in 10 h slots, as presented in the bar plot along with the standard deviation values during 50 h period. The linearly fitted line indicates average doubling frequency as 0.8 day−1. On-chip cell proliferation rate is compared to the bulk petri dish doubling frequency, which is 1.1 day−1. (b) Time lapse, phase contrast microscope images of arrayed single MIA PaCa-2 cells cultured in the microfluidic device. (Multimedia view) [URL: http://dx.doi.org/10.1063/1.4942457.3]
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