Figure 5.

Effects of intrathecal intrathecal administration of NSC23766 (1 mg·mL⁻¹), a selective inhibitor of Rac1, on the bee venomBV‐induced phosphorylation of ERK1/ERK2 and p38 MAPK. The left panels of A and B show representative immunoblotting bands of phosphorylated form and total protein level of ERK1/ERK2 and p38 MAPK. The right panels of A and B show quantitative analysis of the level of phosphorylated form and total protein expression for ERK1/ERK2 and p38 MAPK in group of animals receiving s.c. saline injection (Control, n = 5), s.c. BV injection followed by intrathecal administration of vehicle (saline, BV + Veh, n = 5) and s.c. BV injection followed by intrathecal NSC23766 (BV + NSC, n = 5).The relative density for bands of phosphorylated ERK1/ERK2 and p38 MAPK was normalized to the value of total ERK1/ERK2 and p38 MAPK. The relative density for bands of total ERK1/ERK2 and p38 MAPK was normalized to Tubulin. Data are expressed as mean ± SEM. *P < 0.05, significantly different from Control. #P < 0.05, significantly different from BV + Veh. p‐ERK1/p‐ERK2, phosphorylated ERK; p‐p38, phosphorylated p38 MAPK;