Figure 9.

(+)‐Naltrexone and (+)‐naloxone inhibit LPS‐induced IRF3 activation and IFN‐β production. (A) BV‐2 cells were stimulated with LPS (200 ng·mL⁻¹) and 200 μM of (+)‐naltrexone/(+)‐naloxone for 6 h. Phosphorylation of IRF3 was detected by Western blotting. Five independent experiments were performed (n = 5). *P < 0.05. (B) BV‐2 cells were stimulated with LPS (200 ng·mL⁻¹) and different concentrations of (+)‐naltrexone or (+)‐naloxone. After 24 h treatment, IFN‐β in the supernatant was measured by ELISA. Data are presented as means ± SD (n = 6 in each group).