Figure 3.
The effects of cannabidiol (CBD) are mediated by PPARγ and 5HT1A receptors. The effect of the receptor antagonists GW9662 (A; 100 nM) and WAY100135 (C; 300 nM) on the effects of CBD on BBB permeability. The effect of the PPARγ agonist pioglitazone (B) and 5HT1A receptor agonist 8‐OH‐DPAT (D) applied luminally on BBB permeability. Data are given as mean ± SEM (n represents the total number of inserts measured, based on two to four separate experiments). Statistical analysis was conducted by two‐way anova with post hoc analysis at each time point comparing data against the vehicle control. *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001; vehicle versus CBD. # P < 0.05; vehicle versus CBD + antagonist.