Figure 3.
TG2 involved in cystamine relaxation of mesenteric small arteries. (A) Original traces showing contraction to phenylephrine (Phe; 10 μM) in preparations pretreated with either vehicle or the inhibitor of TG2, monodansylcadaverine, followed by concentration–response curves for either cystamine or the PLC inhibitor, U73122. The bar corresponds to a tension (ΔT) of 4 Nm−1. (B) Average concentration–response curves for cystamine, a TG2 selective non‐cell‐permeable inhibitor, T101; a TG2 selective cell‐permeable inhibitor, LDN 27219; and the suicide substrate, monodansylcadaverine, in arteries contracted with phenylephrine. Arteries were incubated with monodansylcadaverine (100 μM) followed by a washout and then construction of concentration–response curves for (C) cystamine, (D) LDN 27219 or (E) a phospholipase inhibitor, U73122. Monodansylcadaverine pretreatment caused significant rightward shifts in the concentration–response curves for cystamine and LDN 27219. *P < 0.05 versus the control curve (vehicle), n = 6.