Figure 1.

GIP(1–30)NH₂ is a high‐affinity full agonist of the GIP receptor. (A) Alignment of the truncated GIP variants. Human native GIP(1–42) sequence was acquired from National Center for Biotechnology Information Protein Database. The GIP receptor was transiently transfected in COS‐7 cells and used for functional (B) and binding studies (C). (B) cAMP accumulation assay with increased concentrations of native GIP(1–42) and GIP(1–30)NH₂, mean ± SEM, n = 8. (C) Competitive binding with the ¹²⁵I‐GIP(1–42) radioligand displaced by GIP(1–42) and GIP(1–30)NH₂. Data shown are means ± SEM, n = 13.